Coding

Part:BBa_K2216002:Design

Designed by: Zhizhu Zhang   Group: iGEM17_SUIS_Alpha_Shanghai   (2017-10-19)


Malonate-semialdehyde dehydrogenase from Erwinia pyrifoliae DSM 12163


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 375
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 535
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 532


Design Notes

The gene has a EcoRI restriction site GAATTC. We used a silence mutation by changing it to GAATTT.



Source

The gene is from Erwinia pyrifoliae DSM 12163. The gene is coded by Genscript.

References